Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine

ABSTRACT Objective: To evaluate the regeneration of mandibular cartilage defect after implantation of human umbilical cord mesenchymal stem cells (hUCMSC) over platelet rich fibrin (PRF) as scaffold. Material and Methods: 20 male Wistar rats were randomly divided into four experimental groups consisting of: a control group featuring untreated mandibular defects (C), experimental groups whose mandibular defects were implanted with hUCMSC (E1), mandibular defects implanted with PRF (E2), mandibular defects implanted with hUCMSC and PRF scaffold (E3). The subjects were sacrificed after six weeks of observation for immunohistochemical examination in order to evaluate the expression of Ki67, Sox9, FGF 18, type 2 collagen, and aggrecan, in addition to histology examination to evaluate chondrocyte number and cartilage thickness. Data was analyzed with univariate analysis (ANOVA). Results: The implantation of hUCMSC and PRF scaffold proved capable of regenerating mandibular cartilage defect through the expression of FGF 18, Sox9, Ki67, chondrosis counts, type 2 collagen, aggrecan, and cartilage thickness. The regeneration were significantly higher in group E3. Conclusion: Human umbilical cord mesenchymal stem cells in platelet rich fibrin scaffold proved capable of regenerating mandibular cartilage defect.

Comparison of Transforming Growth Factor 1 (TGF-β 1) Expression in Various Lysate Platelet-Rich Fibrin (L-PRF) Concentrations on Human Dental Pulp Stem Cell Differentiation

ABSTRACT Objective: To compare Transforming growth factor-β1 (TGF-β1) expression in various L-PRF concentrations on the hDPSC differentiation process. Material and Methods: hDPSC cell cultures were subjected to serum starvation by reducing FBS levels in the hDPSC culture media. Lysate PRF was obtained from the PRF gel, which was then incubated at 4°C for 24 h. The supernatant was dried, transferred to a 2-ml Eppendorf tube, and stored at −20°C. The evaluation of TGF-β1 expression in 1%, 5%, 10%, and 25% L-PRF samples and 10% FBS (control) during the process of hDPSC differentiation was quantified using an ELISA reader on day 7. The expression of TGF-β1 was subjected to a one-way ANOVA test, followed by Bonferroni's post hoc test with significant values (p<0.05). Results: Significant differences were noted in TGF-β1 expression between 1%, 5%, 10%, and 25% L-PRF and the control group (10% FBS). The highest TGF-β1 expression occurred with 25% L-PRF (0.645 ± 0.048), followed by 10% L-PRF (0.461 ± 0.035), 10% FBS (0.374 ± 0.013), 5% L-PRF (0.275 ± 0.045), and the lowest expression was with 1% L-PRF (0.160 ± 0.045). Conclusion: The best result of TGF-B1 expression in hDPSC differentiation was in the 25% L-PRF group.

Utilização de A-PRF em alvéolo após extração atraumática / Application of A-PRF in alveolus after atraumatic extraction

A fibrina leucoplaquetária autóloga tem sido muito utilizada imediatamente após exodontia devido propriedades benéficas, como aumento na velocidade e na intensidade da vascularização dos tecidos para possibilitar a regeneração dos tecidos periodontais remanescentes operados, bem como o tecido ósseo perdido ou preenchimento do espaço da raiz dentária removida com a finalidade de neoformação óssea, além de diminuir o risco de contaminação pós-operatória de enxertos por utilizar apenas o sangue do próprio paciente sem utilização de aditivos. A-PRF e I-PRF constituem a nova geração de fibrina rica em plaquetas atuando na angiogênese, controle imunológico, liberação de fatores de crescimento e de células mesenquimais. O objetivo do presente relato de caso clínico foi enaltecer meios de preservação de tecido ósseo através de cirurgia minimamente invasiva com uso de extrator dentário, bem como a rápida reconstrução parcial ou total do mesmo com o uso de fibrina rica em plaquetas avançadas (A-PRF) com finalidade de posterior reabilitação local com implante osseointegrado (AU).

Autologous leukoplatelet fibrin has been widely used immediately after extraction due to beneficial properties. Properties such as increased velocity and intensity of tissue vascularization to enable regeneration of the remaining operated periodontal tissues, as well as lost bone tissue or filling of the tooth root space removed with the purpose of bone neoformation² and also for its reduced risk of postoperative grafts contamination as it uses only the patient's own blood without the use of additives. A-PRF and I-PRF constitute the new generation of platelet rich fibrin acting in angiogenesis, immunological control, release of growth factors, and mesenchymal cells. The purpose of the present case report was to enhance means of bone tissue preservation through minimally invasive surgery with the use of a dental extractor, as well as rapid partial or total reconstruction of the same with the use of advanced platelet rich fibrin (A-PRF) with the purpose of later local rehabilitation with osseointegrated implant (AU).